Take the output of anvi-gen-variability-profile, prepare an output for interactive interface.
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anvi-script-snvs-to-interactive -o OUTPUT_DIR \ variability-profile
Then, you can open the interactive interface by running
anvi-interactive --manual-mode \ -p OUTPUT_DIR/profile.db \ --tree OUTPUT_DIR/tree.txt \ --view-data OUTPUT_DIR/view.txt
By default, all variability positions in your variability profile are considered. However, if the input is too large (i.e. more than 25,000 variability positions), the runtime on this program will be very long and the results won’t display well. So, there are several ways to remove variability positions from the input to get under this threshold:
If you still have more positions than you can tell the program to pick a random subset of the input with the parameter
--random followed by a seed integer.
By the default, the output data will use the departure from consensus values. If instead you want to look at the departure from the reference, just add the falg
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__resources__ tag in this file to see an example.