Resources to learn anvi'o and more

Resources below cover a wide range of topics from most basic concepts to most advanced ones to help and inspire any scientist who studies microbial life through ‘omics.

You can click on individual tags visit topic-specific pages, such as metagenomics, to find learning material and exercises on metagenomic read recruitment, genome-resolved metagenomics, genome binning, and refinement, or pangenomics to gain theoretical insights into pangenomics and find tutorials and exercises on how to generate pangenomes and studying them, or hands-on to find all resources that will get your hands dirty with data.

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Tutorials and exercises

Tutorials are the best way to start learning anvi’o. They typically use mock or simple datasets to describe basic concepts and how to accomplish common tasks in anvi’o often with hands-on experience (add more here).

An introduction to Unix Basics of the command line environment, the best friend of any scientist who wishes to work with data.
Basics of metagenomic read recruitment A very simple introduction to metagenomic read recruitment.
A metagenomic read recruitment exercise A mini hands-on exercise is to conduct a simple read recruitment experiment with a mock dataset.
A pangenomics exercise A reproducible pangenomic analysis of seven Vibrio jascida genomes isolated in Woods Hole by the participants of Microbial Diversity Course.
An exercise on single-cell genomics A hands-on tutorial to learn how to integrate single-cell genomics data. It covers functional and taxonomic assignment, pangenomic and phylogenomic analyses, and more.
Anvi'o phylogenomics workflow A comprehensive description of the anvi'o phylogenomics workflow.
An exercise on metabolic reconstruction in anvi'o A hands-on exercise for the metabolism suite of programs in anvi’o. It starts with a section on how to estimate metabolism for a single bacterial isolate, and goes into larger, real-world dataset of metagenome-assembled genomes to demonstrate how to estimate metabolism compute enrichment scores for metabolic modules.
Anvi'o tools for microbial population genetics A theoretical tutorial describing how to characterize SNVs, SCVs and SAAVs with anvi’o, and how to interpret the output.
A primer on anvi'o with the Infant Gut Dataset A tutorial that walks you through some of the most fundamental aspects of anvi'o and its application to a real-world dataset organized in multiple interconnected chapters, which all use the same dataset.
A metagenomics workshop featuring anvi'o Materials from a 2022 workshop on metagenomics in anvi'o, including read recruitment and metabolism estimation.
Another anvi'o workshop on metagenomics Materials and a tutorial from the 2023 iteration of our metagenomics workshop.
Scaling up your analysis with workflows Using publicly available genomes and metagenomes, and anvi'o build in snakemake workflow to contextualize a genome of interest.

Technical write-ups

Articles listed this section offer solutions or discussions regarding matters that are typically more technical than scientific (add more here).

Getting help from the anvi'o community A resource that describes how to find anvi'o programs and understand their utility, how to reach out to anvi'o community, and how to report technical problems.
Anvi'o interactive interface 101 A short tutorial that walks you through the capabilities of the anvi'o interactive interface using an intuitive dataset and _without_ using any of the actual anvi'o functionality.
Anvi'o 'views' demystified Default data views in anvi'o intearctive interface
Data types of anvi'o interactive interface a more detailed description of the interface by demonstrating the data types the interface can work with, and later details of the user interface.
Running remote anvi'o interactive interfaces on your local computer A workaround to run anvi'o on a remote server, and display graphical user interfaces on your local browser seamlessly.
Combining multiple 'omics data types A workflow that describes how to combine metagenomics and metatrasncriptomics data appropriately.
Accessing and including NCBI genomes in 'omics analyses in anvi'o A tutorial that will walk you through the steps of downloading genomes of interest from the NCBI, processing NCBI GenBank files to get anvi’o compatible files, and running the anvi’o snakemake workflow for contigs to generate a contigs database for each of these genomes.
Extract loci from genomes and metagenomes with anvi'o A tutorial on how to use anvi-export-locus to target genomic regions of interest across genomes and/or metagenomic assemblies, and report sequences and/or anvi’o contigs databases for cut loci for downstream analyses.
Combining reference genome annotations with your own in pangenomes Demonstration of a way to incorporate new genomes with reference genomes from NCBI, while maintaining their vetted reference-genome annotations.
Importing VirSorter2 results into anvi'o A workflow (and a script) to import results from VirSorter2, a multi-classifier, expert-guided approach to detect diverse DNA and RNA viruses, into anvi'o databases as a collection of contigs
Integrating AGNOSTOS gene categories into anvi'o projects A workflow to demonstrate how to import AGNOSTOS gene categories into anvi'o databases
Integrating sequence variants and predicted protein structures A theoretical tutorial describing the details of the structure database, how it’s created, and its utility.

Articles, workflows, opinions

These resources often cover key insights into specific topics and can be useful to more advanced users and ‘omics enthusiasts in general (add more here).

The history of metagenomics: An incomplete summary An incomplete history of ideas that shaped integrated genomics and metagenomics to explore microbial fitness in habitats such as the human gut and the oceans.
Visualizing the fate of contigs across metagenomic binning algorithms A visual demonstration of the shortcomings of automatic binning of genomes from complex environments using short-read assemblies.
Binning giant viruses and their close relatives A demonstration of how to reconstruct genomes of giant viruses.
The Ruegeria pomeroyi digital microbe Showcasing our strategy for consolidating and sharing data with a C-CoMP model organism.
Inspecting the genomic link between Archaea and Eukaryota An easy-to-follow diary of resolving a composite Lokiarchaeum genome bin.
An Akkermansia story with MinION long-reads An application of metagenomic long-read sequencing, assembly, correction, and the use of anvi'o pangenomics workflow to benchmark outcomes.

Developer and contributor resources

Here you will find technical documentation to help you learn how to contribute to anvi’o, as a developer or otherwise (add more here).

Updating anvi’o documentation and putting those changes online A tutorial for anyone interested in contributing to the anvi'o help pages.
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