trnaseq-db [artifact]

DB

A DB-type anviā€™o artifact. This artifact is typically generated, used, and/or exported by anviā€™o (and not provided by the user)..

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Provided by

anvi-trnaseq

Required or used by

anvi-convert-trnaseq-database

Description

A tRNA-seq database is an anviā€™o database that contains tRNA sequences predicted from a trnaseq-fasta for a sample, and associated information.

This database is the key output of anvi-trnaseq, which predicts which input sequences are tRNA, clusters them into discrete biological sequences, and predicts positions in the sequences that are the sites of nucleotide modifications. The database therefore contains tables of information produced by each part of the process.

  • Unique sequences predicted to be tRNA, including read counts
  • Primary sequence and secondary structural features (stems and loops) predicted in each unique tRNA
  • Unconserved nucleotides in the primary sequence that differ from expectation
  • Unpaired nucleotides in the stems
  • ā€œTrimmedā€ tRNA sequences, formed from unique sequences only differing by 5ā€™ nucleotides beyond the acceptor stem and 3ā€™ nucleotides of the CCA acceptor region
  • ā€œNormalizedā€ tRNA sequences, formed by dereplicating trimmed tRNA sequences that are 3ā€™ fragments produced by incomplete reverse transcription and by mapping biological tRNA fragments
  • Potentially modified tRNA sequences, formed by clustering normalized tRNA sequences and retaining those clusters that differ by 3-4 nucleotides at potentially modified positions

This database is the key input to anvi-convert-trnaseq-database, which takes one or more databases comprising the samples of an experiment and generates a contigs-db of tRNA seed sequences and profile-db. These can then be displayed and manipulated in anviā€™o like other ā€˜omics data.

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