Oldenburg workshop - March 2025

We are planning a free, in-person workshop and early-career researcher symposium to discuss integrated microbial ‘omics and learn how to use anvi’o. The topics that we will cover during the workshop include,

  • Genomics
  • Metagenomics
  • Genome-resolved metagenomics
  • Pangenomics
  • Phylogenomics
  • Microbial population genetics

If you are interested in attending, please consider applying as soon as possible as we have limited number of seats in the workshop venue, and mark your calendars for March 3-7 2025.

Please feel free to share this page with anyone who may benefit from this workshop, and please note that we will cover travel and accommodation (as long as our funds last), so we strongly encourage applications from institutions with limited resources around the globe.

Feel free to write to any of us if you have any questions.

The application deadline was on Jan 10, 2025 and we are no longer accepting new applications.

The flyer for the workshop.

When and Where

From March 3, 2025 (Monday) to March 7, 2025 (Friday) at the Helmholtz Institute for Functional Marine Biodiversity (HIFMB) in Oldenburg, Germany. Here is a map for you:

Program

In addition to intense hands-on training sessions, this event includes an ECR symposium, during which all of the participants will give lightning talks about their research. We will send the details of lightning talks to each participant upon their invitation.

Here is a very preliminary overview of the workshop content:

Monday 3: Genomics

9:00 - 9:15 Introduction to the workshop faculty & setting
9:15 - 10:15 Introduction to the anvi’o software ecosystem
10:15 - 10:30 Coffee & Tea Break
10:30 - 12:00 The anvi’o interactive interface
12:00 - 13:00 Group lunch at Bar Celona
13:00 - 14:00 Symposium talks
14:00 - 15:30 Genomics (gene calling, functional annotation, taxonomic characterization)
15:30 - 15:45 Coffee & Tea Break
15:45 - 17:30 Genomics, continued

Tuesday 4: Metabolic reconstruction; Pangenomics

9:00 - 10:15 Functions and metabolism (estimation of metabolic potential in genomes and metagenomes)
10:15 - 10:30 Coffee & Tea Break
10:30 - 12:00 Functions and metabolism, continued
12:00 - 13:00 Group lunch at Bar Celona
13:00 - 14:00 Symposium talks
14:00 - 15:30 Phylogenomics (theoretical introduction and hands-on session)
15:30 - 15:45 Coffee & Tea Break
15:45 - 17:30 Phylogenomics, continued

Wednesday 5: Pangenomics; Phylogenomics

9:00 - 10:15 Pangenomics (introduction to pangenomics, and its computational and biological underpinnings)
10:15 - 10:30 Coffee & Tea Break
10:30 - 12:00 Pangenomics (hands-on session)
12:00 - 13:00 Group lunch at Bar Celona
13:00 - 14:00 Symposium talks
14:00 - 15:30 Pangenomics (hands-on session), continued
15:30 - 15:45 Coffee & Tea Break
15:45 - 17:30 Pangenome graphs (theory + hands-on)

Thursday 6: Metagenomics; Genome-resolved metagenomics

9:00 - 10:15 Metagenomic read recruitment (theoretical introduction and hands-on session)
10:15 - 10:30 Coffee & Tea Break
10:30 - 12:00 Metagenomic read recruitment, continued
12:00 - 13:00 Group lunch at Bar Celona
13:00 - 14:00 Symposium talks
14:00 - 15:30 Genome-resolved metagenomics (a discussion on best practices in genome binning and refinement)
15:30 - 15:45 Coffee & Tea Break
15:45 - 17:30 Genome-resolved metagenomics, continued

Friday 7: Microbial population genetics; Scalable bioinformatics

9:00 - 10:15 Microbial population genetics (single-nucleotide variants, single-codon variants, single-amino acid variants, protein structures, and what we can learn from them)
10:15 - 10:30 Coffee & Tea Break
10:30 - 12:00 Microbial population genetics, continued
12:00 - 13:00 Group lunch at Bar Celona
13:00 - 14:00 Symposium talks
14:00 - 15:30 How to use automated, scalable, and reproducible bioinformatics workflows
15:30 - 15:45 Coffee & Tea Break
15:45 - 17:30 Bioninformatics workflows, continued

Workshop materials

The lecture slides for the workshop can be downloaded from this Figshare link.

The table below shows the links to the tutorials used in each workshop session.

Monday Interactive interface https://anvio.org/tutorials/single-cell-genomics-workshop/#visualize-a-sag
Monday Genomics https://anvio.org/tutorials/single-cell-genomics-workshop/#create-and-annotate-a-contigs-database (until ‘Working with Multiple SAGs’ section)
Tuesday Metabolism https://anvio.org/tutorials/fmt-mag-metabolism/
Tuesday Phylogenomics https://anvio.org/tutorials/single-cell-genomics-workshop/#phylogenomics
Wednesday Pangenomics https://anvio.org/tutorials/single-cell-genomics-workshop/#pangenomics
Wednesday Pangenome graphs https://anvio.org/tutorials/pangenome-graphs/
Thursday Metagenomic read recruitment https://merenlab.org/tutorials/read-recruitment/
Thursday Metagenomic binning https://merenlab.org/tutorials/infant-gut/#chapter-i-genome-resolved-metagenomics and https://anvio.org/tutorials/binning-popgen-oral-microbiome
Friday Population genomics https://merenlab.org/tutorials/infant-gut/#chapter-vi-microbial-population-genetics and https://anvio.org/tutorials/binning-popgen-oral-microbiome/#population-genetics
Friday Workflows https://anvio.org/tutorials/scaling-up/

How much does it cost?

We will cover your lunches and coffee/tea breaks. Travel support and accommodation is available upon request while the funds last. The rest is up to you.

We thank the Universitätsgesellschaft Oldenburg e.V. (UGO) and the Alfred-Wegener-Institute (AWI) for their financial support of this event.

The application deadline was January 10, 2025.

List of Faculty

The following individuals from the HIFMB were responsible for organizing, teaching, setting-up and running the workshop.

Name Role
Florian Trigodet Primary Organizer & Faculty
Iva Veseli Primary Organizer & Faculty
Meren Organizer & Faculty
Alexander Henoch Faculty, Teaching Assistant & Coffee Break Manager
Katy Lambert-Slosarska Faculty, Teaching Assistant & Coffee Break Manager
Metehan Sever Teaching Assistant, On-the-fly Bug Fixer
Raïssa Meyer Coffee Break Assistant & Workshop Participant

List of participants

The following 48 individuals attended the workshop.

Full Name University / Institution  
Abigail Turnlund Helmholtz Institute for Functional Marine Biodiversity at the niversity of Oldenburg (HIFMB)
Alberto Scarampi University of Warwick  
Andrian P. Gajigan University of Hawaii at Manoa  
Angela M. Marulanda G. ICBM - Carl von Ossietzky Universität Oldenburg  
Ankish Arya Indian Institute of Information Technology (Allahabad, Prayagraj)  
Archontis Goumagias University Medical Center Groningen (UMCG)  
Balamurugan Sadaiappan United Arab Emirates University  
Berna de Vries University of Vienna  
Carolina Salvador Duque Estrada Federal University of Rio de Janeiro  
Carolina Targon Tiberio University of São Paulo  
Caroline Sophie Winther-Have University of Copenhagen  
Claudia Pogoreutz University of Perpignan Via Domitia  
Emeline Vidal Marine Biological Laboratory  
Fabian Moye Helmholtz Institute for Functional Marine Biodiversity at the Unive sity of Oldenburg (HIFMB)
Fengqing Wang Max Planck Institute for Marine Microbiology  
Garance Gauthier Max Planck Institute for Marine Microbiology  
Ibrahim Cem Özsefil Max Planck Institute for Marine Microbiology  
Joseane Aparecida Marques Helmholtz Institute for Functional Marine Biodiversit at the University of Oldenburg (HIFMB)
Judith Giel Universität Bremen  
Keith Mluleki Dube Stellenbosch University  
Kristina Weinert Max Planck Institute for Marine Microbiology  
Laurice Beatrice Raphaelle dela Pena University of the Philippines  
Manzoor Ahmad United Arab Emirates University  
Mariana Martinez Noriega National Autonomous University of Mexico  
Marianne Lalli University of Helsinki  
Marie-Therese Fischer University of Vienna  
Mertcan Esti Max Planck Institute for Marine Microbiology  
Michael Dills University of Tromsø - The Arctic University of Norway  
Mike Smykala Carl von Ossietzky Universität Oldenburg  
Nathan Ernster University of Innsbruck  
Nikita Rajkumari Indian Institute of Science Education and Research Kolkata, In ia
Nonsikelelo Precios Hlongwa Stellenbosch University  
Paula García Martínez King Abdullah University of Science and Technology  
Pedro Junger Institut de Biologie de l’École Normale Supérieure (IBENS)  
Pranita Mishra Max Planck Institute for Marine Microbiology  
Raïssa Meyer Helmholtz Institute for Functional Marine Biodiversity at the Univ rsity of Oldenburg (HIFMB)
Renato Gamba Romano University of São Paulo / Oceanographic Institute  
Sabrin Abdelghany ZMT Institute  
Samuel Huerten ICBM - Carl von Ossietzky Universität Oldenburg  
Sergio Garcia Manogil Fernandez IE University  
Shivang Pravin Bhanushali University of Turku  
Shiyi Liu Radboud University  
Siyu Mei University of Groningen  
Thomas Klammsteiner University of Innsbruck  
Vladimir Mikryukov University of Tartu  
Xianghui Kong Max Planck Institute for Marine Microbiology  
Xinyu Tang Max Planck Institute for Marine Microbiology  
Yueqing Wang Universität Bremen