Beginner


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Tutorials and exercises

Tutorials are the best way to start learning anvi’o. They typically use mock or simple datasets to describe basic concepts and how to accomplish common tasks in anvi’o often with hands-on experience (add more here).

An introduction to Unix Basics of the command line environment, the best friend of any scientist who wishes to work with data.
Basics of metagenomic read recruitment A very simple introduction to metagenomic read recruitment.
A metagenomic read recruitment exercise A mini hands-on exercise is to conduct a simple read recruitment experiment with a mock dataset.
A pangenomics exercise A reproducible pangenomic analysis of seven Vibrio jascida genomes isolated in Woods Hole by the participants of Microbial Diversity Course.
An exercise on single-cell genomics A hands-on tutorial to learn how to integrate single-cell genomics data. It covers functional and taxonomic assignment, pangenomic and phylogenomic analyses, and more.
Anvi'o phylogenomics workflow A comprehensive description of the anvi'o phylogenomics workflow.
An exercise on metabolic reconstruction in anvi'o A hands-on exercise for the metabolism suite of programs in anvi’o. It starts with a section on how to estimate metabolism for a single bacterial isolate, and goes into larger, real-world dataset of metagenome-assembled genomes to demonstrate how to estimate metabolism compute enrichment scores for metabolic modules.
A primer on anvi'o with the Infant Gut Dataset A tutorial that walks you through some of the most fundamental aspects of anvi'o and its application to a real-world dataset organized in multiple interconnected chapters, which all use the same dataset.
Scaling up your analysis with workflows Using publicly available genomes and metagenomes, and anvi'o build in snakemake workflow to contextualize a genome of interest.

Workshops

Previous and upcoming workshops for a hands-on experience of anvi’o.

Chicago - 2023 Materials and a tutorial from the 2023 iteration of our metagenomics workshop.
Chicago - 2022 Materials from a 2022 workshop on metagenomics in anvi'o, including read recruitment and metabolism estimation.

Technical write-ups

Articles listed this section offer solutions or discussions regarding matters that are typically more technical than scientific (add more here).

Getting help from the anvi'o community A resource that describes how to find anvi'o programs and understand their utility, how to reach out to anvi'o community, and how to report technical problems.
Anvi'o interactive interface 101 A short tutorial that walks you through the capabilities of the anvi'o interactive interface using an intuitive dataset and _without_ using any of the actual anvi'o functionality.
Anvi'o 'views' demystified Default data views in anvi'o intearctive interface
Data types of anvi'o interactive interface a more detailed description of the interface by demonstrating the data types the interface can work with, and later details of the user interface.
Running remote anvi'o interactive interfaces on your local computer A workaround to run anvi'o on a remote server, and display graphical user interfaces on your local browser seamlessly.

Articles, workflows, opinions

These resources often cover key insights into specific topics and can be useful to more advanced users and ‘omics enthusiasts in general (add more here).

The history of metagenomics: An incomplete summary An incomplete history of ideas that shaped integrated genomics and metagenomics to explore microbial fitness in habitats such as the human gut and the oceans.
Visualizing the fate of contigs across metagenomic binning algorithms A visual demonstration of the shortcomings of automatic binning of genomes from complex environments using short-read assemblies.
Inspecting the genomic link between Archaea and Eukaryota An easy-to-follow diary of resolving a composite Lokiarchaeum genome bin.

Developer and contributor resources

Here you will find technical documentation to help you learn how to contribute to anvi’o, as a developer or otherwise (add more here).

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